|
KMID : 1145120210190010101
|
|
Asian Journal of Beauty and Cosmetology
2021 Volume.19 No. 1 p.101 ~ p.108
|
|
|
In Vitro Assessment of the Cytotoxicity of Cuticle Remover Cosmetics
|
|
Kim Ju-Weon
An Eun-Joo
Kim Min-Kyeong
Kim Jeong-Hee
|
|
|
Abstract
|
|
|
Purpose : Information regarding the hazards of cuticle removers is scarce, and product safety reports are also insufficient. This study aimed to assess the safety of cuticle remover cosmetics.
Methods : This study evaluated the safety of the top three commercially sold cuticle removers, Blue cross, Zero cleanser, and Flower vita, which are all widely used in nail care. Human nail matrix cells were isolated and cultured in a defined medium. The toxic effect of cuticle removers on the cultured human nail matrix cells, HS68, and HaCaT, were identified via MTT-assay. The cuticle remover cosmetics were diluted to the following concentrations: 0.5, 1.0, 1.75, 2.5, 10, 20, and 25 μg/mL.
Results : All three cuticle removers showed significant cytotoxicity to the human nail matrix, HS68, and HaCaT cells in a dose-dependent manner. The viability of cells treated with 10 μg/mL of Blue Cross and Zero cleanser was below 10%. Conversely, Flower Vita treatment at concentrations of 0.5-20 μg/mL did not affect the viability (above 70%) of the nail matrix cells, HS68, and HaCaT.
Conclusions : Among the cuticle removers tested, Flower Vita showed the lowest cytotoxic effect in the nail matrix, HS68, and HaCaT cells, proving its safety. This study supports that nail matrix cells are developing as an evaluation method for nail damage.
|
|
|
KEYWORD
|
|
|
Nail cosmetic, Cuticle remover, Nail matrix cell, Potassium hydroxide, MTT assay
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|